Fertility

Screens for Erectile Dysfunction

Model	Post-coital Administration of the test item in female rats
Parameter	Number of implantations Number of corpus lutea Number of Live/Dead Fetuses Anti-fertility Activity Body Weight Change
End points	Estrogen Progesterone

Assessment of Galactogogue Potential

Model	Evaluation of Galactogogue (Lactogenic) activity
Test system	Mouse mammary epithelium cell line (HC11)
Method	Identification of non-cytotoxic concentrations of Test item by MTT assay. Treatment of cells with Test item at non-cytotoxic concentrations Effect on mammosphere formation by phase contrast microscopy Effect on casein production by western blotting.
End points	No. of mammospheres Casein production