In Vitro Model for identification of anti-rhinitic compounds
Model	Evaluation of anti-rhinitic activity
Test system	Human nasal epithelial cell line (RPMI 2650) Human airway epithelial cell line (A549)
Method	Treatment of cells with test compounds Induction of inflammatory conditions by treatment with appropriate stimulant (HDM extract/LPS) Assessment of TGF-ß2 secretion in RPMI 2650 cell line Assessment of IL-6 & IL-8 in A549 cell line
End points	Quantitation of inflammatory cytokines