Gastrointestinal

Gastrointestinal

Model	In vitro model for digestive stimulants
Test system	Estimation of digestive enzyme activities using biochemical assays: Pancreatic & intestinal homogenates of rat Commercially available enzymes
Method	Evaluation of test compound’s effect on the activities of digestive enzymes of gastrointestinal tract
End points	Pancreatic amylase, Lipase, Trypsin & Chymotrypsin Intestinal Sucrase, Lactase & Maltase

Model	Cell free biochemical assays
Test system	Pancreatic & intestinal homogenates of wistarrat Commercially available enzymes
Method	Preparation of pancreatic and intestinal homogenates from wistar rat Treatment with test item Pancreatic amylase, Lipase, Trypsin & Chymotrypsin Intestinal Sucrase, Lactase & Maltase
End points	Digestive stimulatory potential of test item reflected by enhanced enzyme activitie

Anti-ulcer potential	Cel lfree assays–anti-oxidantandant acid Adenocarcinoma cell line(AGS)
Test system	Humana denocarcinoma cell line(AGS)
Method	Anti-oxidant -DPPH/ABTS assay ?Acid neutralizing capacity -Titrimetric assays with NaOH Cytoprotection in AGS cell line -Treatment of AGS cells with test item (5 concentrations) -Exposure of cells to acidic medium (pH-3)/Indomethacin/ethanol -Effect of test item on survival of cell
End points	Anti-ulcer potential of test item reflected by anti-oxidant, antacid and cytoprotective activitie