dabur

Est.1979

Providing Integrated Research Solutions in Preclinical Biology

Modules

In Vitro Pharmacology

Gastrointestinal

ModelIn vitro model for digestive stimulants
Test systemEstimation of digestive enzyme activities using biochemical assays:
  • Pancreatic & intestinal homogenates of rat
  • Commercially available enzymes
MethodEvaluation of test compound’s effect on the activities of digestive enzymes of gastrointestinal tract
End points
  • Pancreatic amylase, Lipase, Trypsin & Chymotrypsin
  • Intestinal Sucrase, Lactase & Maltase
Model
  • Cell free biochemical assays
Test system
  • Pancreatic & intestinal homogenates of wistarrat Commercially available enzymes
MethodPreparation of pancreatic and intestinal homogenates from wistar rat Treatment with test item Pancreatic amylase, Lipase, Trypsin & Chymotrypsin Intestinal Sucrase, Lactase & Maltase
End points
  • Digestive stimulatory potential of test item reflected by enhanced enzyme activitie

 

Anti-ulcer potential
  • Cel lfree assays–anti-oxidantandant acid
  • Adenocarcinoma cell line(AGS)
Test system
  • Humana denocarcinoma cell line(AGS)
MethodAnti-oxidant -DPPH/ABTS assay ?Acid neutralizing capacity -Titrimetric assays with NaOH Cytoprotection in AGS cell line -Treatment of AGS cells with test item (5 concentrations) -Exposure of cells to acidic medium (pH-3)/Indomethacin/ethanol -Effect of test item on survival of cell
End points
  • Anti-ulcer potential of test item reflected by anti-oxidant, antacid and cytoprotective activitie
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