dabur

Est.1979

Providing Integrated Research Solutions in Preclinical Biology

Modules

In Vitro Pharmacology

Immunomodulation

We offer a comprehensive range of in vitro & ex vivo models for identification of immunostimulant & immunosuppressant potential of molecules in vitro /ex vivo models for immunomodulators

in vitro Model for identification of Immunomodulators
ModelChemokine & cytokine profiling for identification of immunomodulators
Test systemMurine bone marrow derived dendritic cell cultures
Methods
  • Generation of Dendritic cells in vitro
  • Characterization of dendritic cells
  • Profiling of potential of test item for modulation of cytokines & chemokines
  • Validation with immunosuppressant & immunostimulatory molecules
End pointsQuantitation of TNF- a, IL-1- ß, MIP-1- a , IL-6 ,RANTES, IL -12, IFN– ?, CCL- 22, IP -10, IL -4, IL -10 & TGF-ß

in vitro Model for identification of Immunomodulators
ModelPhagocytosis assay for identification of immunostimulants
Test systemMacrophage cell line (RAW 264.7)
Methods
  • Treatment of macrophages with test compounds
  • Evaluation of immunostimulatory effect of test compounds exhibited by internalization of particulate material (Zymosan) by macrophage cells
End pointsColorimetric estimation of Zymosan uptake by cells

in vitro Model for identification of Immunomodulators
ModelNatural Killer (NK) cells based assay for identification of immunostimulants
Test systemEffector cells – Murine splenocytes (NK cells) Target cells – Murine lymphoma cell line (YAC-1)
Methods
  • Treatment of NK cells with test compounds
  • Co-incubation of NK cells with YAC-1 cells
  • Assessment of immunostimulatory effect of test compounds on NK cells mediated lysis of YAC-1 cells
End pointsEstimation of NK cells mediated targeted lysis of YAC-1 cells by MTT assay or fluorescence based methods

 

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