dabur

Est.1979

Providing Integrated Research Solutions in Preclinical Biology

Modules

In Vitro Pharmacology

Fertility

ModelPost-coital Administration of the test item in female rats
Parameter
  • Number of implantations
  • Number of corpus lutea
  • Number of Live/Dead Fetuses
  • Anti-fertility Activity
  • Body Weight Change
End points
  • Estrogen
  • Progesterone

 

ModelEvaluation of Galactogogue (Lactogenic) activity
Test systemMouse mammary epithelium cell line (HC11)
Method
  • Identification of non-cytotoxic concentrations of Test item by MTT assay.
  • Treatment of cells with Test item at non-cytotoxic concentrations
  • Effect on mammosphere formation by phase contrast microscopy
  • Effect on casein production by western blotting.
End points
  • No. of mammospheres
  • Casein production
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