dabur

Est.1979

Providing Integrated Research Solutions in Preclinical Biology

Differentiator

Alternates To Animal Testing

IN VITRO/EX VIVO MODELS: ALTERNATIVES TO ANIMAL TESTING

Dabur Research Foundation offers various in vitro or ex vivo models serving as alternatives to animal testing by focusing on the 3R’s principle.

These methods accelerate the screening process by decreasing time taken for screening and hence are more economical than animal testing. At DRF, we assure to offer efficient, accurate, reliable and economic solutions as the alternative to the animal experimentation.

We offer following models as alternatives to animal testing:

S. NoPharmacological relevanceBioassayTherapeutic area
1.Anticancer screeningMTT assay in cancer cell linesOncology
2.Synergistic/additive/antagonistic anticancer activityMTT assay in cancer cell linesOncology
3.Normal cell toxicityMTT assay/morphogical characterizationOncology
4.Dermal safetyHuman Fibroblasts assayDermatology
5.Mechanism of uptake of drugs1)  Tracking of drug delivery by TEM
2)  Cellular uptake by HPLC
Oncology
6.Dermal cosmetic products for
anti-ageing potential
Primary human dermal Fibroblasts assayDermatology
7.Dermal cosmetic products for
skin-whitening potential
B16 Melanoma cell line assayDermatology
8.Systemic hypersensitivity potentialComplement activation assayOncology
9.Allergy/Anti-allergy potentialHistamine estimation in Mast cell-lineAllergy
10.DiabetesGlucose uptake assayMetabolic Diseases
11.Hair growth potentialHuman dermal papilla cells assayDermatology
12.Anti-inflammatory potential1)  Murine Dendritic cells
2)  Murine Macrophage cell line
3) Human Monocytic cell line
Inflammation
13.Immunomodulatory potential1)  Murine Dendritic cells
2)  Murine Macrophage cell line
3)  Human Monocytic cell line
Immunomodulation
14.AngiogenesisPrimary endothelial cells assayOncology
15.Antioxidants1) SOD assay
2) Catalase assay
3) GSH assay
4) MDA assay
Other
16.PhototoxicityNRU assay in NIH-3T3 cell lineDermatology
17.Chemotherapy induced toxicityLiver, heart, lung, kidney and intestinal cell linesOncology
18.Chemotherapy induced hematotoxicityCFU-GM assay
CFU-E assay
CFU-MK assay
Oncology
19.DigestionAmylase activity assay
Lipase activity assay
Trypsin activity assay
Chymotrypsin activity assay
Disaccharidases assay
(Sucrase, Lactase, Maltase)
Gastrointestinal
20.Wound healing potentialDermal fibroblast cell lineDermatology
21.Anti-osteoarthritis/Rheumatoid arthritisSynovial sarcoma cell line
Chondro sarcoma cell line
Inflammation
22.Efficacy of targeted drugsTarget overexpression in using cell linesOncology
23.Intestinal permeabilityCaCO-2 cell monolayers-metGastrointestinal
24.Protein BindingUltrafiltration Method 
25.SolubilityDMSO Precipitation (Kinetic solubility Determination) 
26.In vitro permeabilityPAMPA 
27.Analgesic activityRat whole blood assay for PGE2 estimationMetabolic Diseases
28.LeucodermaMelanogenesis stimulatory assay in B16 melanoma cell linesDermatology
29.Anti-osteoporotic activityAlkaline phosphatase activity in osteoblast-like cell linesMetabolic Diseases
30.Anti-cirrhotic activity1)  Primary hepatocytes
2)  Hepatic stellate cells
Metabolic Diseases
31.Photoprotective effectUV induced damage using fibroblasts cell linesDermatology
32.RhinitisNasal epithelial cell linesInflammation
33.AsthmaBronchial smooth cell lines/Airway epithelium cell linesAllergy
34.HypoallergenicityDendritic cells based assayAllergy
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