Modules

In Vitro Pharmacology

In Vitro Cosmetic Screens

We offer a range of models designed to undertake Dermo – cosmetic assessment of products for a range of indications. These include the following:

In Vitro Cosmetic Screen

Skin health

Screening models for assessment of skin rejuvenation potential
Model	In vitro cell based models
Test System	Human dermal fibroblast and keratinocyte cell lines
Method	Determination of non-cytotoxic concentrations of test item Treatment with non-cytotoxic doses of test item Quantitation of biomarkers for skin health in vitro
End points	Fibroblast proliferation Improvement of skin Texture — Collagen synthesis — Elastin synthesis — MMP expression Improvement of Skin moisture Hyaluronic acid synthesis

Phagocytosis

Testing of Products with anti ageing & anti wrinkling potential
Model	In vitro cell based models
Test system	Human dermal fibroblast cell line
Method	Determination of non-cytotoxic concentrations of test item Treatment with non-cytotoxic doses of test item Challenge with UV-or H2O2 (oxidative stress) to induce premature senescence Comparison of anti ageing potential of test item with reference drugs/ products
End points	Cellular viability and proliferation Protection against UV/H2O2 induced damage Levels of extracellular matrix proteins (collagen, elastin, etc.) MMP expression Hyaluronic acid level

Skin lightening / depigmentation

Testing of Products with anti ageing & anti wrinkling potential
Model	In vitro cell based models
Test system	Human dermal fibroblast cell line
Method	Determination of non-cytotoxic concentrations of test item Treatment with non-cytotoxic doses of test item Challenge with UV-or H2O2 (oxidative stress) to induce premature senescence Comparison of anti ageing potential of test item with reference drugs/ products
End points	Cellular viability and proliferation Protection against UV/H2O2 induced damage Levels of extracellular matrix proteins (collagen, elastin, etc.) MMP expression Hyaluronic acid level

Testing of Products with anti ageing & anti wrinkling potential
Model	In vitro cell based models
Test system	Human dermal fibroblast cell line
Method	Determination of non-cytotoxic concentrations of test item Treatment with non-cytotoxic doses of test item Challenge with UV-or H2O2 (oxidative stress) to induce premature senescence Comparison of anti ageing potential of test item with reference drugs/ products
End points	Cellular viability and proliferation Protection against UV/H2O2 induced damage Levels of extracellular matrix proteins (collagen, elastin, etc.) MMP expression Hyaluronic acid level

Testing of Products with potential to reduce oxidative stress in skin
Model	Cell free assay In vitro cell based models
Test system	Human dermal fibroblast and keratinocyte cell lines
Method	Cell free assay-Free radical scavenging effect by DPPH/ABTS assay Cell based assay Determination of non-cytotoxic concentrations of test item Treatment with non-cytotoxic doses of test item Challenge with UV-or H2O2 to induce oxidative stress Validation with antioxidant molecules or client defined molecules
End points	Cell free assay-Free radical scavenging effect Cell based assay Lipid peroxidation Total glutathione levels Super oxide dismutase/catalase enzyme activity Levels of reactive oxygen species