- Fit for purpose methods (small and larger molecules)
- bioanalytical method validation (small and larger molecules)
- Quantitative determination of biomarker in plasma and tissues
- Chemical derivatization techniques to improve chromatographic behavior and ionization efficiency
- Extraction technique : protein precipitation, Liquid liquid extraction, Solid Phase Extraction
- Impurity profiling
- Metabolite identification
Herb-Drug Interaction Background Herb-Drug interaction is a situation in which constituent/s of herbal extract or product affects the activity of a drug when administered together. Millions of people today use herbs either as food or in the form of medicine along with prescription and nonprescription medications. Although considered natural and safe, many of these herbs can interact with other medications, causing either potentially dangerous side effects and/or reduced benefits from the medication. Interactions between herbs and drugs may increase or decrease the pharmacological or toxicological effects of either component. Due to the limitation of availability of experimental data in this area, there is a huge need to perform herb-drug interaction studies with herbal products. Assay offered for Herb-Drug Interaction In-Vitro methods
Model |
In-vitro model for drug-drug or herb-drug interaction |
Test System |
Human Liver Microsomes or recombinant enzymes |
Method |
Co-incubation of herb-drug or drug-drug with CYP enzymes |
Quantitation method |
HPLC-UV or LC/MS/MS or Fluorescence reader |
End Point |
Metabolite formation |
Deliverables |
Percent inhibition, IC50 determination |
Model |
In-vitro model for drug-drug or herb-drug interaction |
Test System |
Liver cell lines |
Method |
Co-incubation of herb-drug or drug-drug with CYP enzymes |
Quantitation method |
HPLC-UV or LC/MS/MS or Fluorescence
reader |
End Point |
Metabolite formation |
Deliverables |
Percent inhibition, IC50 determination |
In-Vivo methods
Model |
Pharmacokinetics |
Test System |
Rodents |
Method |
Co-administration of drug with herbal product |
Quantitation method |
HPLC-UV or LC/MS/MS |
End Point |
PK parameter evaluation of drug |
Deliverables |
Impact of herb on PK profile of drug |