• Extraction procedure optimization
  • HPLC Fingerprinting of Herbal Extract
  • Marker identification and characterization
  • Quantitation of marker in herbal extract and product
  • Specification setup of herbal marker
  • Quantitation of marker in biological matrix (blood, plasma etc.)
Test system Human MSCs (for batch characterization)
  • Receipt of MSCs cell suspension (batch wise)
  • Staining of cells fluorescent-tagged antibodies
  • Acquisition of stained cells on flow cytometer (Guava Technologies).
End points Percentage expression of positive markers – CD105, CD73, and CD90 Percentage expression of negative markers – CD14, CD19, CD34, CD45 and HLA-DR