Est.1979
Providing Integrated Research Solutions in Preclinical Biology
IN VITRO/EX VIVO MODELS: ALTERNATIVES TO ANIMAL TESTING
Dabur Research Foundation offers various in vitro or ex vivo models serving as alternatives to animal testing by focusing on the 3R’s principle.
These methods accelerate the screening process by decreasing time taken for screening and hence are more economical than animal testing. At DRF, we assure to offer efficient, accurate, reliable and economic solutions as the alternative to the animal experimentation.
We offer following models as alternatives to animal testing
| S. No | Pharmacological relevance | Bioassay | Therapeutic area |
| 1. | Anticancer screening | MTT assay in cancer cell lines | Oncology |
| 2. | Synergistic/additive/antagonistic anticancer activity | MTT assay in cancer cell lines | Oncology |
| 3. | Normal cell toxicity | MTT assay/morphogical characterization | Oncology |
| 4. | Dermal safety | Human Fibroblasts assay | Dermatology |
| 5. | Mechanism of uptake of drugs | 1) Tracking of drug delivery by TEM 2) Cellular uptake by HPLC |
Oncology |
| 6. | Dermal cosmetic products for anti-ageing potential |
Primary human dermal Fibroblasts assay | Dermatology |
| 7. | Dermal cosmetic products for skin-whitening potential |
B16 Melanoma cell line assay | Dermatology |
| 8. | Systemic hypersensitivity potential | Complement activation assay | Oncology |
| 9. | Allergy/Anti-allergy potential | Histamine estimation in Mast cell-line | Allergy |
| 10. | Diabetes | Glucose uptake assay | Metabolic Diseases |
| 11. | Hair growth potential | Human dermal papilla cells assay | Dermatology |
| 12. | Anti-inflammatory potential | 1) Murine Dendritic cells 2) Murine Macrophage cell line 3) Human Monocytic cell line |
Inflammation |
| 13. | Immunomodulatory potential | 1) Murine Dendritic cells 2) Murine Macrophage cell line 3) Human Monocytic cell line |
Immunomodulation |
| 14. | Angiogenesis | Primary endothelial cells assay | Oncology |
| 15. | Antioxidants | 1) SOD assay 2) Catalase assay 3) GSH assay 4) MDA assay |
Other |
| 16. | Phototoxicity | NRU assay in NIH-3T3 cell line | Dermatology |
| 17. | Chemotherapy induced toxicity | Liver, heart, lung, kidney and intestinal cell lines | Oncology |
| 18. | Chemotherapy induced hematotoxicity | CFU-GM assay CFU-E assay CFU-MK assay |
Oncology |
| 19. | Digestion | Amylase activity assay Lipase activity assay Trypsin activity assay Chymotrypsin activity assay Disaccharidases assay (Sucrase, Lactase, Maltase) |
Gastrointestinal |
| 20. | Wound healing potential | Dermal fibroblast cell line | Dermatology |
| 21. | Anti-osteoarthritis/Rheumatoid arthritis | Synovial sarcoma cell line Chondro sarcoma cell line |
Inflammation |
| 22. | Efficacy of targeted drugs | Target overexpression in using cell lines | Oncology |
| 23. | Intestinal permeability | CaCO-2 cell monolayers-met | Gastrointestinal |
| 24. | Protein Binding | Ultrafiltration Method | |
| 25. | Solubility | DMSO Precipitation (Kinetic solubility Determination) | |
| 26. | In vitro permeability | PAMPA | |
| 27. | Analgesic activity | Rat whole blood assay for PGE2 estimation | Metabolic Diseases |
| 28. | Leucoderma | Melanogenesis stimulatory assay in B16 melanoma cell lines | Dermatology |
| 29. | Anti-osteoporotic activity | Alkaline phosphatase activity in osteoblast-like cell lines | Metabolic Diseases |
| 30. | Anti-cirrhotic activity | 1) Primary hepatocytes 2) Hepatic stellate cells |
Metabolic Diseases |
| 31. | Photoprotective effect | UV induced damage using fibroblasts cell lines | Dermatology |
| 32. | Rhinitis | Nasal epithelial cell lines | Inflammation |
| 33. | Asthma | Bronchial smooth cell lines/Airway epithelium cell lines | Allergy |
| 34. | Hypoallergenicity | Dendritic cells based assay | Allergy |